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KMID : 0613820010110010043
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Journal of Life Science
2001 Volume.11 No. 1 p.43 ~ p.47
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Evidences for structural changes of {TEX}$Pro^{28}${/TEX}→Leu mutant tryptophan synthase α-subunit
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Abstract
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A mutant tryptophan synthase α-subunit, where Pro28 was replaced with Leu, tends to be expressed in recombinant E. coli. CD and fluorescence spectra of this protein indicate some changes in secondary and tertiary structure. Wild type protein was more or less affected by {TEX}$Ca^{2+}${/TEX} ion in regards of the fluorescent properties of its native, unfolded and intermediate forms, but the mutant protein was not at all. The dramatic structural changes may be related to the aggregation of this mutant protein.
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